P-26: Histomorphological Study of Rat Prostate Gland

Background: Rat prostate gland which is used in various investigation has considerable morphological differences with other mammals. There are few reports about its structure. So, this study was carried on for determining of histomorphological structure of rat prostate. Materials and Methods: Five healthy adult male rats with average weight 20 ± 180 g and approximately 3 - 3.5 months age were studied. Samples were taken from different lobes, and 5- 6 μ sections were made using paraffin embeding method. Sections were stained with H and E and PAS. The histometrical studies were done using digital Dino-Lite lens and Dino- capture software. Results: The macroscopic results showed that the rat prostate consisted of four lobes including: anterior, ventral, dorsal, and lateral lobes, which lateral lobe divided 2 sublobes types 1 and 2. Microscopicopical observation showed that prostate parenchyma consisted of secretory ducts and secretory alveoli-tubular units. The secretory alveolar units have many folds but these folds are vary in different lobes, so that the anterior lobe has the maximum folded secretory units and they are spread across the entire lob but they were located in peripheral zone of other lobes (p ≥ 0.05). Conclusion: The alveoli cells have different staining intensity, so that the alveoli cells of the lateral lobe 1 is more acidophilic than other lobes. The proportion of secretory units to secretory duts varied in different lobes, so that the dorsal lobe has maximum proportion. Tubular units are usually free of folds and mostly are located in the center of the lobes. The results showed that the epithelial secretory units has twice thickness compare to secretory duct.